Preparation of Immunoglobulin Y from Egg Yolk Using Ammonium Sulfate Precipitation and Ion Exchange Chromatography

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Preparation of immunoglobulin Y from egg yolk using ammonium sulfate precipitation and ion exchange chromatography.

The objective of this study was to develop an economical, simple, and large-scale separation method for IgY from egg yolk. Egg yolk diluted with 9 volumes of cold water was centrifuged after adjusting the pH to 5.0. The supernatant was added with 0.01% charcoal or 0.01% carrageenan and centrifuged at 2,800 x g for 30 min. The supernatant was filtered through a Whatman no. 1 filter paper and the...

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A simple method for isolating chicken egg yolk immunoglobulin using effective delipidation solution and ammonium sulfate.

Chicken egg yolk immunoglobulin (IgY) is a superior alternative to mammalian immunoglobulin. However, the practical application of IgY in research, diagnostics, and functional food is limited due to complex or time-consuming purification procedures. The objective of this study was to develop a simple, safe, large-scale separation method for IgY from egg yolk. Egg yolk was diluted with 6-fold de...

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ژورنال

عنوان ژورنال: Poultry Science

سال: 2007

ISSN: 0032-5791

DOI: 10.1093/ps/86.2.400